I have been completely frustrated at work lately. I can't seem to get anything to work! I don't know how much of this will make sense to most of you, but I will try to explain anyway. I am in the process of putting together an expression vector that will allow us to express mutant genes in frog embryos. The problems is I can't seem to get the pieces I need out of their current vectors to put into this new one! Not only am I frustrated, but the boss man is frustrated with me. The protocols I am using are standard and not difficult, but they just don't work for whatever reason. I am not doing anything incorrectly; they just aren't working. Molecular biology is so frustrating sometimes. I keep doing the same experiments over and over again, and they just don't work. There is no reason that I can see for this stuff to not work. A guy I work with is having the same problem with his stuff right now, so I don't feel quite as bad. I still dread having to talk with Dr. Weeks about it because then I feel quite incompetent.